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Trm1p is shown to be required for MRE1-dependent methanol-inducible gene expression.
Chromatin immunoprecipitation assays reveal that Trm1p binds to five methanol-inducible promoters upon methanol induction but does not bind in glucose-grown cells.
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This indicates the type of evidence that supports the existence of the protein.
The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x x 1.
The algorithm is described in the ISO 3309 standard.
Expression of the -acting methanol response elements (MREs), MRE1 and MRE2 are present in the promoter of the dihydroxyacetone synthase gene.
Formaldehyde is situated at the branching point of the assimilation and dissimilation pathways.
DAS catalyzes the first reaction of the assimilation pathway by fixing formaldehyde with xylulose-5-phosphate.
The molecular mechanism of methanol-inducible gene expression, however, has yet to be elucidated.
When methylotrophic yeasts grow on methanol as the sole carbon source, peroxisomes massively proliferate and can occupy up to 80% of the intracellular volume.